A semiquantitative bioassay for relative virulence of Agrobacterium tumefaciens strains on Bryophyllum daigremontiana.

نویسندگان

  • S L Minnemeyer
  • R Lightfoot
  • A G Matthysse
چکیده

An assay to determine the relative virulence of wild-type and mutant strains of Agrobacterium tumefaciens on leaves of Bryophyllum daigremontiana has been developed. This assay is reproducible, is easy to learn, is not time consuming, and requires little space. The relative virulence of cellulose-minus mutants of A. tumefaciens was investigated with this assay. Some of these mutants were unaltered in virulence, while others showed a marked reduction in virulence.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Time required for tumor induction by Agrobacterium tumefaciens.

Cellulose-minus mutants of Agrobacterium tumefaciens retain virulence but can be removed from wound sites by washing with water. Washing of Bryophyllum diagremontiana leaves inoculated with a cellulose-minus mutant was used to determine the minimum time the bacteria must be present for tumor induction. This time was 4 to 8 h.

متن کامل

A Method for Eliminating the Contribution of Agrobacterium tumefaciens to Studies of Nucleic Acid Biosynthesis in Crown Gall Tumors.

A procedure is described that permits the use of radiotracer methods for examining crown gall tumors in the presence of the initiating agent Agrobacterium tumefaciens. On the basis of sensitivity testing using the disc diffusion technique and the minimum inhibitory concentration procedure and incorporation of radioactive phosphorus into nucleic acids, it was determined that A. tumefaciens was m...

متن کامل

Attachment of Agrobacterium tumefaciens to carrot cells and Arabidopsis wound sites is correlated with the presence of a cell-associated, acidic polysaccharide.

An early step in crown gall tumor formation involves the attachment of Agrobacterium tumefaciens to host plant cells. A. tumefaciens C58::A205 (C58 attR) is a Tn3HoHo1 insertion mutant that was found to be avirulent on Bryophyllum daigremontiana and unable to attach to carrot suspension cells. The mutation mapped to an open reading frame encoding a putative protein of 247 amino acids which has ...

متن کامل

Quantitative aspects of transformation of virulence in Agrobacterium tumefaciens.

In the last decade, a number of bacterial species were shown to possess the ability to transmit morphological and biochemical characters to deficient strains. Tumor-inducing ability or virulence in crown-gall bacteria is such a biochemical character, and the acquisition of virulence is here taken to be the acquisition by avirulent strains of bacteria of the ability to synthesize a tumor-inducin...

متن کامل

Agrobacterium tumefaciens VirC2 enhances T-DNA transfer and virulence through its C-terminal ribbon-helix-helix DNA-binding fold.

Agrobacterium tumefaciens VirC2 stimulates processing of single-stranded T-DNA that is translocated into plants to induce tumor formation, but how VirC2 functions is unclear. Here, we report the 1.7-A X-ray crystal structure of its trypsin-resistant C-terminal domain, VirC2(82-202), which reveals a form of the ribbon-helix-helix (RHH) DNA-binding fold contained within a single polypeptide chain...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of bacteriology

دوره 173 23  شماره 

صفحات  -

تاریخ انتشار 1991